Hello, dear friend, you can consult us at any time if you have any questions, add WeChat: daixieit

Quiz 3 - Chem214

(Summer 2022)

1)  2pts  Draw an epimer of D-Idose.

1pt How many stereoisomers does D-Idose have?

2)  3pts.  Draw the disaccharide sucrose.  Indicate what type of glycosidic linkage it contains.

3.) You have isolated the enzyme milleniase from the rare Y2K bug. This enzyme cleaves the first two numbers off of four digit dates. You flex your skills as an enzymologist and  come up with the following graph of initial velocity as a function of substrate                    concentration:

a)        2 pts From the graph, estimate Km and Vmax?

b)        2 pts Draw a double reciprocal plot of this enzyme in the presence of a competitive inhibitor.

c) 2pts Explain what happens to the Vmax and Km.

4.) 3 pts Draw the pH activity curve for a protein that contains an active site OH (pKa = 10) and the protein is active with the proton OFF.

1 pt At what pH would you do your enzyme assays?

5.) 4pts  What kind of glycosidic linkage makes up glycogen?  Draw these repeating       carbohydrate linkages.  What disaccharide is produced when glycogen is hydrolyzed?