Quiz 2 – Chem214 (Summer 2022)
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Quiz 2 – Chem214
(Summer 2022)
1.) 4 pts What is a protein protease? Give an example of two protein protease and describe its specificity.
2.) 2 pts. Give the Michaelis Menton equation.
3.) 4 pts An enzyme has an initial rate of 45 μmol/min at a substrate concentration of .020 µM and a Km of 3 mM. What is the enzymes maximal rate?
4.) 2 pts An enzyme has a Km1 = 245M for S1 and a Km2 = 0.95mM for S2 . Which substrate does the enzyme have a higher affinity for? S1 or S2 (circle one).
5.) 4 pts An enzyme has an initial rate of 45 μmol/min at a substrate concentration of .020 µM and a Km of 3 mM. What is the enzymes maximal rate?
6.) Consider the peptide: Val-Ala-Glu-Asp-Met-Lys-Arg-Pro-Ala
a) 2 pt What would be the amino acid released during the first round using Carboxypeptidase A?
b) 2 pt How many peptide fragments would be produced from a Trypsin cleavage?
2022-08-10