Prerequisite: 3 credit hours in college-level biology

Microbiology 4000.01 is an upper-level GE course that provides a scientific look into the biology of microorganisms and how they impact humans and everyday life. Through both lecture and lab components, students will learn aspects of microbial growth, metabolism, diversity, genetics, pathogenesis, human immune defenses , and scientific methods for culturing and identifying microbes.

Instructor Information

Course Instructors: Dr. Tammy Bullwinkle

Email (preferred method): bullwinkle.1@osu.edu use “Micro 4000” in the subject line

Office: 376 Biological Sciences Building, 484 W 12thAve

Phone: 292-5867

Office Hours: Wed 1:00 – 2:00p and Thurs 10:30a – 12:00p or by appointment

Dr. Maggie Wetzel

Email (preferred method): wetzel.160@osu.edu use “Micro 4000” in the subject line

Office: 318 Biological Sciences Building, 484 W 12thAve

Phone: 292-8257

Office Hours: Tues & Wed 1:00 – 2:00p or by appointment

Teaching Assistants: See Carmen for the updated list of TAs and contact information.

Attendance and Participation

Lecture section 24281: MWF @ 10:20am – 11:15pm in Hitchcock Hall 131

Lecture section 23987: MWF @ 3:00pm – 3:55pm in Independence Hall 100

In-person attendance in lecture is expected and necessary for success. Students are responsible for any notes, activities, discussions, and announcements given during lecture. Livestream is available with pre-registration. Limit 5 lectures.

Lectures are not recorded. Lecture slides and materials will be available on Carmen and Top Hat. If you have a situation that might cause you to miss an entire week of lecture, discuss it with Dr. Bullwinkle or Dr. Wetzel ASAP.

See lecture schedule for more details.

Attendance is Required at in-person, proctored quizzes and exams. See exam policies (p. 11) for more information.

Lab: Biological Sciences 3rd  Floor Labs

Attendance in the laboratory is mandatory. Lab is held once weekly, in-person on your scheduled day/time/room. It is necessary for course completion to attend the labs. Students may miss up to 3 labs (excused or unexcused) ; the 4th absence will result in failure in Microbiology 4000. Due to the nature of this course, a make-up lab is not possible, but depending on the circumstances, students can earn credit back for up to 2 missed labs. See the lab absence policy for more details.

Should in-person lectures or labs be canceled, we will notify you as to which alternative methods of teaching will be offered (such as Zoom) to ensure continuity of instruction for this class. Communication will be via email and Carmen Canvas.

Credit Hours and Work Expectations: This is a 4-credit-hour course. According to Ohio State policy, students should expect around 4 hours per week of time spent on direct instruction (e.g. instructor content, lab content, and Carmen activities) in addition to 6 hours of homework (e.g. reading, assignment preparation, and studying) to receive a grade of (C) average. These expectations are based on a typical 15-week term.

Materials and Technologies Needed for this Course

Lecture Textbook: “Microbiology: The Human Experience” 3rd ed. by Foster et al. W.W. Norton (+ online tools).

The textbook and courseware for lecture are being provided via Carmen Books, an OSU program that offers access to certain electronic textbooks for a fraction of the cost. The fee for this material is included as part of tuition and is

listed as Carmen Books fee on your Statement of Account. Materials will be available on or before the first day of class. There is no need to wait for financial aid or scholarship money to purchase your textbook.

Unless you choose to opt-out of the program, you do NOT need to purchase any lecture materials at the bookstore. For more information or information on how to opt out, please visit the Carmen Books website.

Access the electronic textbook and courseware are through links on Carmen.

Laboratory Manual:

Printed copy of “Basic and Practical Microbiology Lab Manual” (Newly Revised First Edition), Cognella Academic Publishing. The paperback manual can be ordered directly from Cognella, Order Here.

Course Technology: For help with your password, university email, Carmen, or any other technology issues, questions, or requests, contact the Ohio State IT Service Desk. Standard support hours are available at https://it.osu.edu/help, and support for urgent issues is available 24/7.

Self-Service and Chat support: go.osu.edu/IT

Email: [email protected]

Phone: 614-688-4357(HELP)

Accessibility Help: 614-292-8000

MICROBIOLOGY 4000 LEARNING OUTCOMES

Successful students will be able to …

1.   Describe the basic morphology of bacteria, their growth requirements, and how they adapt and reproduce. 1,2

2.   Explain how environmental factors affect the culturing of microbes in a laboratory setting. 1,2

3.   Understand the diversity of metabolism that exists in bacteria and describe the unique metabolic ways used only in the prokaryotic world.1

4.   Describe bacterial genetics with the emphasis on two main processes responsible for the diversity in the bacterial world: mutations and bacterial gene transfer.1

5.   Describe the basic morphology of viruses, how they reproduce and cause diseases. 1

6.   Apply appropriate aseptic techniques when completing any exercise in lab.2

7.   Correctly carry out  basic  microbiology laboratory techniques  including, Gram staining, production of pure cultures, production of dilution series and enumeration of microbes, inoculation of various culture media, the filter disk method of testing bacterial susceptibility to various agents, and use of the ELISA test.2

8.   Correctly and safely use any equipment needed to carry out exercises in lab.2

9.   Demonstrate how to identify unknown bacteria using tests, media, and techniques introduced in lab.2

10. Explain  how  unknown  bacteria  are  identified through  submission  of a  lab  report that  includes  a flow  chart  and interpretation of results.2

11. Describe Biofilm and explain how it is formed.1,2

12. Explain how microbes are involved both constructively and destructively in foods. 1,2

13. Explain microbial symbiosis based on observations made in lab.2

14. Describe some of the normal microbiota of the nose, mouth, and skin. 1

15. Understand infection and disease control. Understand the preventative techniques necessary to limit the spread of bacteria and viruses.1

16. Describe and explain various ways to control the growth of microbes.1,2

17. Describe how to track the spread of a simulated disease, and how to determine the index case. 1,2

18. Describe the system of innate or non-specific immunity.1

19. Describe adaptive immunity; understand how it is acquired and how it protects us.1

20. Describe how microbes overcome the host body’s immune mechanisms and how they are transmitted. 1

21. Explain  several  aspects  of  immunity  based  on  observations  made  in  lab.   Including:  phagocytosis,  precipitin, agglutination, and complement fixation.1,2

22. Describe the etiological agent, pathogenesis, symptoms and treatment of microbial diseases of the Respiratory system and the Digestive system.1

1Lecture goal

2Lab goal