Bio 99 Exam 2 - 2021: SUMMER S2: MOLECULAR BIO
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Bio 99 Exam 2 - 2021: SUMMER S2: MOLECULAR BIO
Bio 99 Exam 2 - 2021
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Figure 1:
The domain that cuts the DNA on the crRNA bound strand is
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CRH
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Question 3 1 / 1 pts |
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Figure 1: The domain that cuts the DNA on the PAM strand is
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Question 4 0 / 1 pts |
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Figure 1: When we say the DNA was "cleaved", it means that there is a break in bonding induced by a
covalent, nucleophile H, nucleophile H, nucleotide
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Question 5 1 / 1 pts |
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Figure 2: What is the purpose of the green section of the plasmid? (Amp)
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Question 6 1 / 1 pts |
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Figure 2: What is the purpose of the lightblue section of the plasmid? (pMB1 ori) |
to allow for DNA replication
to allow only bacteria containing plasmid to survive
to allow cutting sites so genes can be ligated in
to catalyze the reaction of non-fluorescent substrate into fluorescent product
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Question 7 1 / 1 pts |
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Figure 2: What is the purpose of the MCS?
◎ to allow only bacteria containing plasmid to survive
0 to allow for DNA replication |
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Question 8 |
1 / 1 pts |
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Figure 3: In the FRET experiment shown here, we would the molecule to induce the FRET system.
0 emit, red |
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Question 9 0 / 1 pts |
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Figure 3:
In a FRET experiment if the donor molecule (HNH in the Doudna talk) has an excitation wavelength of 250 nm, and an emission wavelength of 285 nm; what would be the best "target excitation" for the FRET acceptor (the molecule we are trying to see if HNH is close to). |
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Question 10 1 / 1 pts |
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Figure 4:
The experimental goal was to get GFP into the bacteria by genetic engineering. We introduce the plasmid by using . _________________
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2023-09-03